In continuation of our effort to let you know some of the routine equipments/instruments used for research, this time Rameshwor Pandit from Yeungnam University, South Korea presents two key instruments used in his Organic Synthesis Lab: NMR system and HPLC. These instruments are widely used in research and routine analysis laboratories for variety of applications.
(1) NMR Spectroscopy
This system consists of mainly four parts (1) superconducting magnet (2) detector and amplifier (3) console and (4) spectrum display.
Varian VNS 300 MHz
At the beginning, sample solution is prepared in deuterated solvent. Next, the computer program is opened (above is Varian VNS 300 MHz) and set some of the parameters like temperature, spinning. As soon as sample is inserted into the magnet, the system is locked to a particular deuterated solvent. The Varian system works on automated shimming while Bruker type of system works on mechanical shimming. Now the sample is scanned setting some acquisition parameters like no. of scans, time, sample FID. As the sample is scanned, computer shows spectrum which is processed to get NMR spectrum as shown in computer display (below).
